Journal: Poultry Science

Article Title: Efficacy of a plant-produced infectious bronchitis virus-like particle vaccine in specific pathogen-free chickens

doi: 10.1016/j.psj.2023.102953

Figure Lengend Snippet: SDS-PAGE (A) and Western blot (B) of plant-produced spike protein (synthetic construct mIBV-S2P-NDV-F TM/CT ) coinfiltrated at a 4:1 ratio with the NDV matrix protein. Lane 1: molecular weight marker; Lane 2: plant-expressed empty pEAQ-HT vector; Lane 3: purified live QX-like IBV strain ck/ZA/3665/11; Lanes 4 to 7: Fractions 3 to 6 extracted in PBS buffer; Lanes 8 to 11: Fractions 3 to 6 extracted in Bicine buffer; Lanes 12 to 15: Fractions 3 to 6 extracted in Tris buffer.

Article Snippet: The fractions containing the highest level of S protein expression as confirmed by SDS-PAGE and Western blot were pooled (i.e., those for mIBV-S2P-NDV-F TM/CT :Matrix processed in PBS buffer), dialyzed in 1× PBS using 3500 mW CO Slide-A-Lyzer Dialysis Cassettes (ThermoFisher Scientific, Waltham, MA), and stabilized with 15% (w/v) trehalose dehydrate (Sigma-Aldrich).

Techniques: SDS Page, Western Blot, Produced, Construct, Molecular Weight, Marker, Plasmid Preparation, Purification